The use of ultra-high efficiency filter in biological laboratory construction
For the basic requirements of the ultra-high efficiency filter for the construction of the biosafety laboratory, the environmental parameters are as follows. Compared with the outside of the laboratory, the inside of the laboratory maintains a negative pressure. There are several different types of laboratory construction specifications for reference.
Level 1 Biosafety Protection Laboratory
Laboratory construction safety equipment and personal protection 1. Generally, there is no need to use special safety equipment such as biological safety cabinets. 2. The staff should wear work clothes and protective glasses during the experiment. 3. Workers should wear gloves when they have broken skin or rash on their hands.
Special requirements for laboratory design and construction
1. Each laboratory should have a hand-washing sink, which should be located near the exit.
2. The inner surface of the laboratory enclosure structure should be easy to clean. The ground should be non-slip and seamless, and no carpet should be laid.
3. The surface of the test bench should be impermeable, corrosion-resistant, and heat-resistant.
4. The furniture in the laboratory should be firm. For easy cleaning, a table (rack) of biological waste containers should be kept between various furniture and equipment.
5. If the laboratory has windows that can be opened, screen windows should be installed.
Secondary Biosafety Protection Laboratory
1. Safety equipment and personal protection
1.1 Operations that may produce pathogenic microbial aerosols or splashes should be carried out in a biological safety cabinet (Class II biological safety cabinet is appropriate) or other physical suppression equipment, and personal protective equipment should be used.
1.2 The handling of high-concentration or large-volume infectious materials must be carried out in a biological safety cabinet (Class II biological safety cabinet is appropriate) or other physical suppression equipment, and personal protective equipment should be used. The centrifugation of the above materials can be carried out in the laboratory if sealed centrifuge rotors or safety centrifuge cups are used, and they are only opened and closed in the biological safety cabinet and loaded with infectious materials.
1.3 When the operation of microorganisms cannot be carried out in the biological safety cabinet and external operations must be taken, in order to prevent the splashing or atomization of infectious materials, facial protection devices (goggles, masks, personal respiratory protection products or other protective equipment) must be used. Splash protection equipment).
1.4 Protective clothing such as work clothes or overalls should be worn in the laboratory. When leaving the laboratory, protective clothing must be taken off and left in the laboratory. It is not allowed to go out in a dress, let alone take it home. The used work clothes should be disinfected in the laboratory first, and then uniformly washed or discarded.
1.5 Wear gloves when your hands may come into contact with infectious materials, contaminated surfaces, or equipment. If spills or spills of infectious materials may occur, two pairs of gloves should be worn. Do not leave the laboratory wearing gloves. After the work is completely finished, the gloves can be removed. Disposable gloves must not be washed and reused.
2. Special requirements for laboratory design and construction
2.1 The biological safety protection secondary laboratory must meet the requirements of each paragraph in 6.1.2 of this standard.
2.2 Facilities for implementing various disinfection methods should be set up, such as autoclaves, chemical disinfection devices, etc. to treat waste.
2.3 Eyewash devices should be provided.
2.4 The laboratory door should be locked and can be closed automatically.
2.5 The exit of the laboratory should have a luminous indicator.
2.6 The laboratory should have no less than 3 to 4 ventilation times per hour.
3. Biosafety protection laboratory
1. Safety equipment and personal protection
1.1 Class II or above biological safety cabinets must be installed in the laboratory.
1.2 All operations involving infectious materials should be carried out in a biological safety cabinet. When such operations have to be carried out outside the biological safety cabinet, comprehensive protective measures of personal protection and the use of physical restraint equipment must be adopted.
1.3 Personal protective equipment must be used when conducting infectious tissue culture and operations that may produce infectious aerosols.
1.4 When the aerosol cannot be safely and effectively restricted within a certain range, respiratory protection devices should be used.
1.5 Before entering the laboratory work area, the staff should wear back-opening work clothes or other protective clothing in the special changing room (or buffer room). The work clothes must be taken off after work, and the work clothes are not allowed to leave the laboratory. Work clothes that can be reused must be disinfected and then washed.
1.6 Must wear gloves when working (two pairs are appropriate). Disposable gloves must be disinfected before being discarded.
1.7 The laboratory must be equipped with effective disinfectants, eye cleansers, or physiological saline, and be easy to access. Can be equipped with emergency medicines.
2. Special requirements for laboratory design and construction
2.1 Site selection
The biosafety protection laboratory can be set up in the same building with other houses, but it must be a separate area. This area is separated from public corridors or public places by isolation doors.
2.2 Layout of laboratory construction
a) The core area of the biosafety protection laboratory includes the experimental room and the buffer room connected to it.
b) The buffer room forms a channel into the experiment room. Two interlocking doors must be set. When one of the doors is opened, the other door is automatically closed. If an electric interlocking device is used, both doors must be openable when the power is off. The second dressing can be done in the buffer room.
c) When the ventilation system of the laboratory is not equipped with automatic control devices, the area of the buffer room should not be too large, and should not exceed one-eighth of the area of the experimental room.
d) The installation location of the level II or level III biological safety cabinet should be far away from the entrance of the laboratory, avoid the area where the staff frequently walks, and is conducive to the formation of airflow pattern from the "clean" area to the "contaminated" area.
2.3 Envelope structure
a) The inner surface of the enclosure structure of the laboratory (including the buffer room) must be smooth, corrosion-resistant, waterproof, and easy to disinfect and clean. All gaps must be reliably sealed.
b) All doors in the laboratory can be closed automatically.
c) Except for observation windows, no windows are allowed. The observation window must be a sealed structure, and the glass used is not broken glass.
d) The ground should be free of leakage, smooth but not slippery. Do not use floor tiles and terrazzo on the ground with gaps.
e) The corners between the ceiling, floor, and walls are all arc-shaped and reliably sealed, and insects and mice should be prevented from entering the foot of the wall during construction.
2.4 Ventilation and air conditioning
